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. 2019 Sep 19;10:4272. doi: 10.1038/s41467-019-12080-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Characterization of BG505 SOSIP-I53-50NP assembly and cryo-EM structure. a Representative SEC chromatogram (left) of assembled BG505 SOSIP-I53-50NP (red) overlaid with BG505 SOSIP-I53-50A.1NT1 (gray) and reducing SDS-PAGE (right) of pooled BG505 SOSIP-I53-50NP fractions. b Raw NS-EM micrograph of pooled fractions corresponding to BG505 SOSIP-I53-50NPs. The white bar corresponds to 200 nm. c Segmentation of the sharpened cryo-EM map at high contour level showing details of NP core (left) and Gaussian filtered map at low contour levels showing poorly resolved SOSIP trimers (right). d Details of I53-50A.1NT1 (left) and I53-50B.4PT1 (right) showing the predicted model (top) and cryo-EM map (bottom). Root-mean-square deviation (RMSD) are shown below each component. e Low-pass-filtered cyro-EM map and pseudo-atomic model of BG505 SOSIP-I53-50NP with detail of the linker separating I53-50A.1NT1 and BG505 SOSIP. Source data are provided as a Source Data file