Fig. 6.

Epitope mapping of BG505 and ConM SOSIP-I53-50NP-induced (N)Ab responses. a Color coding for b–e. b–e Statistical differences between two groups were determined using unpaired two-tailed Mann–Whitney U-tests (*p < 0.05; **p < 0.01; ***p < 0.001). b Ratio of midpoint neutralization titers of several BG505 virus mutants relative to the parental BG505 virus is plotted (RID₅₀). Mutants shown are BG505 with glycans knocked in at position 241 and 289 (BG505 241KI+289KI), position 241 (BG505 241KI), position 289 (BG505 289KI), or a glycan knocked out at position 611 (BG505 611KO). Epitope mapping was only performed for sera from rabbits that had an ID₅₀ > 100 (n = 7 individual rabbits, for BG505 SOSIP recipients; n = 4 individual rabbits for BG505 SOSIP-I53-50NP recipients). See also Supplementary Table 3. Horizontal bars indicate the median. c Residual binding of bNAbs CH01, PGT128, VRC01, 8ANC195, ACS202, 35O22, and non-NAb RM19R after incubation of sera (n = 8 individual rabbits) with immobilized BG505 SOSIP in ELISA (left) and surface model of BG505 SOSIP (gray) with the corresponding Ab footprints highlighted. Bars indicate the median. d Midpoint autologous ConM neutralization in the absence of a blocking agent (no blocking), with ConM SOSIP added (+ConM SOSIP) or with ConM SOSIP that has the V1V2-loop of BG505 (+ConM SOSIP (V1V2 BG505)). Statistical differences between the three groups (n = 5 individual rabbits) was determined using a Friedman test followed by a Dunn’s multiple comparisons test (***p < 0.001; ****p < 0.0001). e Residual binding of bNAbs VRC01, PGT128, CH01, PG16, and gl-PG9 after incubation of sera with immobilized ConM SOSIP in ELISA. Bars indicate the median (n = 5 individual rabbits). Source data are provided as a Source Data file