Table 2.
Cryo-EM data collection, refinement, and validation statistics
| BG505 SOSIP-I53-50NP (EMDB-20261) (PDB 6P6F) |
|
|---|---|
| Data collection and processing | |
| Magnification | 38,168 |
| Voltage (kV) | 300 |
| Electron exposure (e−/Å2) | 46.6 |
| Defocus range (μm) | 1.2–5.1 |
| Pixel size (Å) | 1.31 |
| Symmetry imposed | I1 |
| Initial particle images (no.) | 3996 |
| Final particle images (no.) | 3590 |
| Map resolution (Å) | 4.5 (0.143) |
| FSC threshold | |
| Map resolution range (Å) | 4.4–6 |
| Refinement | |
| Initial model used (PDB code) | NA |
| Model resolution (Å) | NA |
| FSC threshold | |
| Model resolution range (Å) | NA |
| Map sharpening B factor (Å2) | −149 |
| Model composition | |
| Non-hydrogen atoms | 0 |
| Protein residues | 351 |
| Ligands | 0 |
| B factors (Å2) | NA |
| Protein | |
| Ligand | |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.017 |
| Bond angles (°) | 1.586 |
| Validation | |
| MolProbity score | 0.5 |
| Clashscore | 0 |
| Poor rotamers (%) | 0 |
| EMRinger score | 1.55 |
| Ramachandran plot | |
| Favored (%) | 99.7 |
| Allowed (%) | 0 |
| Disallowed (%) | 0 |