Figure 3.

Sensitivity to Congo Red and Lysing Enzymes. (A) Mycelia cultured for 1 day were suspended in sodium phosphate buffer (10 mM, pH 6.0) containing 0.8 M NaCl and 10 mg/ml Lysing Enzymes. After 1, 2, and 4 h, protoplasts were counted under a microscope. Error bars represent the standard deviation calculated from three replicates. (B) Growth rates after 3 days on CDE medium at the indicated concentrations of Congo Red. Diameter of the colonies grown on CDE medium without Congo Red was considered as 100%. Error bars represent standard deviations calculated from three replicates. In both panels, different letters indicate significant differences within each condition by Tukey’s test (p < 0.05).