Figure 5.
Visualization of AG and GAG in the cell wall by staining with AGBD-GFP and lectin (soybean agglutinin). Conidia (5.0 × 105) of the wild-type (WT), ΔagsAΔagsBΔagsC (AGΔ), ΔagsAΔagsBΔagsCΔsphZΔugeZ (AG-GAGΔ), and ΔsphZΔugeZ (GAGΔ) strains were inoculated into 500 μl of CDE liquid medium and incubated at 30°C for (A) 0, (B) 3, (C) 6, and (D) 9 h with shaking (1,200 rpm). At each time point, the cells were dropped on a glass slide, fixed with 4% (w/v) paraformaldehyde, stained with AGBD-GFP and soybean agglutinin-Alexa Fluor 647 conjugate (100 μg/ml each), and observed under a confocal laser-scanning microscope (×1,000). Scale bars, 20 μm.