Figure 8.

Mycelial aggregation in the presence of (A) acetylated GAG or (B) urea. (A) The amino groups of ethanol-precipitated GAG were acetylated with acetic anhydrate. Mycelial suspension of the AG-GAGΔ strain (25 μl) was added to a mixture of 450 μl of 100 mM sodium phosphate buffer (pH 7.0) and 25 μl of the 1.5-vol. EtOH fraction prepared from AGΔ (acetylated or not). (B) Mycelial suspension of the AG-GAGΔ strain (25 μl) was added to a mixture of 450 μl of 100 mM sodium phosphate buffer (pH 7.0) containing 0, 1, 2, 4, or 8 M urea, and 25 μl of the 1.5-vol. EtOH fraction prepared from the AGΔ strain. Samples were incubated at 30°C for 1 h with shaking and examined under a stereomicroscope (magnification, ×8).