Table 2.

PCR primers used in this study.

Purpose	Primer name	Sequence (5′ to 3′)
sphZ, ugeZ disruption
	sphZ+ugeZ-LU	TCTCCATAGTGTTCACCA
	sphZ+ugeZ-LL + Ade	ATATACCGTGACTTTTTAGCACAACATTGGAGCTACT
	sphZ+ugeZ-RU + Ade	AGTTTCGTCGAGATACTGCGCGTTGTCATATTTGCAAG
	sphZ+ugeZ-RL	AGGGCTCAGAATACGTATC
	sphZ+ugeZ-AU	AGTAGCTCCAATGTTGTGCTAAAAAGTCACGGTATATCATGAC
	sphZ+ugeZ-AL	TTGCAAATATGACAACGCGCAGTATCTCGACGAAACTACCTAA
Quantitative PCR
	agsA-RT-F	CAAACCTGGAGAGACGCGAT
	agsA-RT-R	CGAGGGTATTCGCAAGTGTTG
	agsB-RT-F	GAACTTTGTCGCGGTCATCCTTCAG
	agsB-RT-R	CCAAGGGAGGTAGTAGCCAATG
	agsC-RT-F	TTGGAGACGGACCATCACTG
	agsC-RT-R	GTTGCAGGTCTCGTTGTACTC