TABLE 1.
Genome features, sequencing statistics, and accession numbers of the 11 conspecific Streptomyces strains
| Strain or replicon | Illumina sequencing information |
Oxford Nanopore sequencing information |
Replicon size (bp)c | Genome size (bp) | Total no. of CDSa | G+C content (%) | TIRb (kb) | GenBank accession no. | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| No. of reads (approx coverage [×]) | SRA accession no. | No. of reads (approx coverage [×]) | N50 of raw reads (kb) | Flow cell type(s) | Sequencing kit(s) | Base caller | SRA accession no. | |||||||
| RLB1-8 | 15,381,622 (320) | SRR9661592 | 655,482 (150) | 4.3 | FAH18893 (9.5), FAH18988 (9.5), FAH24488 (9.5) | sqk-lsk308 | albacore_2.0.2 | SRR9710048 | 11,765,340 | 11,765,340 | 10,635 | 70.2 | 357 | CP041650 |
| RLB1-9 | 18,329,970 (115) | SRR9661591 | 88,694 (41) | 7.6 | FAF19789 (9.4) | sqk-lsk308 | albacore_0.8.4 | SRR9710047 | 11,940,408 | 12,200,709 | 10,838 | 70.2 | 311 | CP041654 |
| pRLB1-9.1 | 154,158C | 175 | 69,0 | CP041653 | ||||||||||
| pRLB1-9.2 | 106,143L | 111 | 68.7 | 24 | CP041652 | |||||||||
| RLB3-5 | 3,196,108 (67) | SRR9661590 | 144,521 (56) | 7.1 | FAH24352 (9.5), FAH29240 (9.4) | sqk-lsk308, sqk-lsk108 | albacore_2.0.2 | SRR9710050 | 11,898,970 | 11,898,970 | 10,731 | 70.2 | 365 | CP041651 |
| RLB3-6 | 3,274,272 (68) | SRR9661589 | 299,155 (52) | 4.6 | FAF19789 (9.4) | sqk-lsk308 | guppy_0.3.0 | SRR9710049 | 12,338,263 | 12,448,281 | 11,255 | 70.1 | 587 | CP041602 |
| pRLB3-6.1 | 110,314C | 101 | 70.6 | CP041601 | ||||||||||
| RLB3-17 | 3,976,622 (83) | SRR9661596 | 202,455 (50) | 5.6 | FAF19789 (9.4) | sqk-lsk308 | guppy_0.3.0 | SRR9710052 | 12,023,175 | 12,023,175 | 10,934 | 70.2 | 451 | CP041610 |
| S1A1-3 | 3,243,184 (68) | SRR9661595 | 198,567 51) | 5.2 | FAF19789 (9.4) | sqk-lsk308 | guppy_0.3.0 | SRR9710051 | 12,042,091 | 12,042,091 | 10,920 | 70.2 | 393 | CP041611 |
| S1A1-7 | 3,504,210 (73) | SRR9661594 | 533,299 (73) | 2.8 | FAF19789 (9.4) | sqk-lsk308 | guppy_0.3.0 | SRR9710054 | 11,713,151 | 12,005,504 | 10,580 | 70.3 | 513 | CP041604 |
| pS1A1-7.1 | 292,353C | 252 | 69.7 | CP041603 | ||||||||||
| S1A1-8 | 3,191,318 (66) | SRR9661593 | 780,962 (53) | 1.1 | FAF19789 (9.4) | sqk-lsk308 | guppy_0.3.0 | SRR9710053 | 12,036,971 | 12,036,971 | 10,918 | 70.2 | 394 | CP041612 |
| S1D4-14 | 2,794,454 (58) | SRR9661598 | 2,066,754 (344) | 3.2 | FAF19789 (9.4) | sqk-lsk308 | guppy_0.3.0 | SRR9710056 | 11,723,487 | 11,934,498 | 10,591 | 70.2 | 369 | CP041607 |
| pS1D4-14.1 | 112,196L | 118 | 68.7 | 0 | CP041605 | |||||||||
| pS1D4-14.2 | 98,815C | 138 | 69.1 | CP041606 | ||||||||||
| S1D4-20 | 3,327,172 (69) | SRR9661597 | 1,424,402 (255) | 4.1 | FAF19789 (9.4) | sqk-lsk308 | guppy_0.3.0 | SRR9710055 | 11,851,257 | 12,245,276 | 10,742 | 70.2 | 373 | CP041609 |
| pS1D4-20.1 | 394,019L | 329 | 69.1 | 68 | CP041608 | |||||||||
| S1D4-23 | 3,543,760 (74) | SRR9661599 | 400,348 (61) | 3.5 | FAF19789 (9.4) | sqk-lsk308 | guppy_0.3.0 | SRR9710057 | 12,057,712 | 12,057,712 | 10,971 | 70.2 | 421 | CP041613 |
a
As determined through automatic annotation by the NCBI Prokaryotic Genome Annotation Pipeline. CDS, coding sequences.
b
TIR, terminal inverted repeat.
c
L/C, linear (L) or circular (C) replicon configuration, as predicted by the assembler and tested by pulsed-field gel electrophoresis (not shown).