Figure 3. Bach2 silencing facilitates Th22 cell differentiation.

CD4⁺ T cells isolated from peripheral blood were transfected as si-Bach2 or scramble siRNA control (si-NC). (A) Representative flow cytometry results of Th22 frequency in transfected CD4⁺ T cells. (B) Expression of Th22-associated transcription factor AHR in transfected CD4⁺ T cells was analyzed by qRT-PCR. (C) The mRNA expression of IL-22 in transfected CD4⁺ T cells was analyzed by qRT-PCR. (D) The levels of IL-22 in transfected CD4⁺ T cells supernatant was analyzed by ELISA. Data were presented as mean ±SD (n=3). *P<0.05 vs non-CHD; ^#P<0.05 vs si-NC. Abbreviations: NC, negative control; non-CHD, angiographic exclusion of CHD.