Skip to main content
. 2019 Sep 20;39(9):BSR20190986. doi: 10.1042/BSR20190986

Figure 5. MiR-31 facilitates Th22 cell differentiation by targeting Bach2.

Figure 5

Naive CD4+ T cells were transfected with miR-31 mimic or Lv-Bach2, Th22 differentiation was induced by 10 ng/ml IL-1β, 30 ng/ml IL-6, 20 ng/ml IL-23, 400 nM FICZ and 10 μM TGF-β receptor inhibitor Galunisertib. (A) Bach2 expression in CD4+ T cells was determined by qRT-PCR and Western blotting. (B) Th22 frequency in CD4+ T cells was determined by flow cytometry. (C,D) The expressions of AHR (C) and IL-22 (D) in CD4+ T cells were analyzed by qRT-PCR. (E) IL-22 level in CD4+ T cells was analyzed by ELISA. Data were presented as mean ± SD (n=3). *P<0.05 vs control; #P<0.05 vs mimic NC+Th22 culture; &P<0.05 vs mimic NC+Lv-NC+Th22 culture.