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| Gene | Forward (5’–3’) | Reverse (5’–3’) |
|---|---|---|
| β-actin | CCGCGAGTACAACCTTCTTG | TGACCCATACCCACCATCAC |
| Aggrecan | ATGGCATTGAGGACAGCGAA | GCTCGGTCAAAGTCCAGTGT |
| Collagen II | GCCAGGATGCCCGAAAATTAG | CCAGCCTTCTCGTCAAATCCT |
| P53 | CCTTAAGATCCGTGGGCGT | GCTAGCAGTTTGGGCTTTCC |
| P16 | TACCCCGATACAGGTGATGA | TACCGCAAATACCGCACGA |
