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. 2019 Sep 13;10:2209. doi: 10.3389/fimmu.2019.02209

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Copyright © 2019 Battin, De Sousa Linhares, Paster, Isenman, Wahrmann, Leitner, Zlabinger, Steinberger and Hofer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Interaction of NRP1 and mNRP1 with complement split products C4d, C3d, and iC3b. (A) Flow cytometric analysis of BW cells transduced to express high levels of human NRP1. Interaction of indicated CSPs (20 μg/ml each) with BW control cells (open histograms) and BW cells expressing NRP1 (gray histograms). Expression of NRP1 was verified with a monoclonal NRP1 antibody. (B) Interaction of plate-bound CSPs (465 nM each) with soluble recombinant human NRP1-immunoglobulin fusion protein (rh-NRP1-Ig) and complement receptor Ig fusion protein (rh-CRIg-Ig) analyzed in an ELISA-based assay. (C) Binding of murine NRP1 (mNRP1) mAb and recombinant human CSPs (rh-C4d, ih-iC3b, and ih-C3d) to BW control cells (open histograms) and BW cells expressing mNRP1 (gray histograms). Data shown is representative of two independently performed experiments.