Figure EV1. Sequential digestion increases the number of identified unique residue pairs in a seven‐protein mixture.

Links per fraction and gain for sequential digestion and the control experiments composed by an experiment using trypsin alone in four replicates and individual digestions with trypsin, AspN, chymotrypsin and GluC. Trypsin yields the higher number of links per sample followed by sequential digestion and individual digestions. However, sequential digestion yields the largest number of unique residue pairs when combining the data.