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. 2019 Sep 13;10:1015. doi: 10.3389/fphar.2019.01015

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Copyright © 2019 Orejudo, Rodrigues-Diez, Rodrigues-Diez, Garcia-Redondo, Santos-Sánchez, Rández-Garbayo, Cannata-Ortiz, Ramos, Ortiz, Selgas, Mezzano, Lavoz and Ruiz-Ortega

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IL-17A-expressing cells in human hypertensive nephropathy. (A) IL-17A expressing cells detected by immunohistochemistry with anti-IL-17A antibody. Figure shows a representative image of human biopsies. (B) Characterization of IL-17A-expressing cells by double immunofluorescence for IL-17A, labeled by an Alexa 633 (red) secondary antibody and for CD4 (for CD4⁺/Th17 cells) or for γδT lymphocytes, both labeled with an Alexa 488 (green) secondary antibody. Nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI; blue). The figure shows a confocal microscopy analysis of a representative patient out of 20 cases studied with similar results.