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. 2019 Sep 13;10:1015. doi: 10.3389/fphar.2019.01015

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Copyright © 2019 Orejudo, Rodrigues-Diez, Rodrigues-Diez, Garcia-Redondo, Santos-Sánchez, Rández-Garbayo, Cannata-Ortiz, Ramos, Ortiz, Selgas, Mezzano, Lavoz and Ruiz-Ortega

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IL-17A-expressing cells in the kidney of rats with experimental hypertension. (A) The presence of IL-17A and γδ T lymphocytes was detected by immunohistochemistry. Figure shows a representative image. (B) Double immunofluorescence for IL-17A, labeled with an Alexa 633 (red) secondary antibody, and CD4 (for CD4⁺/Th17 cells) labeled with an Alexa 488 (green) secondary antibody. Nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI; blue). (C) IL-17A protein levels were evaluated in total protein renal extracts by ELISA. The figure shows a confocal microscopy analysis of a representative rat of four studied with similar results. *p < 0.05 vs control.