Skip to main content
. 2019 Jul 9;47(17):e97. doi: 10.1093/nar/gkz585

Figure 3.

Figure 3.

Functional comparison of CRY2/CIB1 PA-Cre2.0 with Mag PA-Cre. (A) Comparison of PA-Cre2.0 and Mag PA-Cre activity at different expression levels. HEK293T cells were transfected with either 1 μg of each indicated Cre system (KM69 or KM91) and 1 μg DIO-EGFP reporter (1:1 ratio), or with 50 ng each Cre system and 450 ng reporter (1:9 ratio). Samples were treated with dark or 4 h light at 24 h, before quantifying at 48 h. Graph indicates average and range of two independent experiments. (B) Comparison of PA-Cre2.0 and Mag PA-Cre responses to a 4 s light pulse. HEK293T cells were transfected with 1 μg of PA-Cre2.0 (KM69) and 1 μg DIO-EGFP or loxP-STOP-loxP-EGFP reporter (1:1), or 50 ng of Mag PA-Cre (KM91) and 450 ng of each reporter (1:9). Samples were treated with dark or light as in (A) and quantified at 48 h. Graph indicates average and range of two independent experiments.