Figure 6.

PTGS transmission from L1 rootstocks to 6b4 scions requires RDR6 but not DRM2 in 6b4. (A) GUS activity and GUS siRNA accumulation in scion leaves of plants of the indicated genotypes grown under short day conditions for eight weeks after grafting. GUS activity is expressed as fluorescent units per minute per microgram of total proteins quantified by Bradford. RNA was extracted from a bulk of four plants. Ethidium bromide staining is shown as loading control. Distinct boxes indicate that the samples were separated by tracks that are not relevant for this work. The original blot is presented in Supplementary Figure S4. (B) Percentage of DNA methylation at representative CHH and CHG methylation-sensitive enzymes in the 5′ and 3′ regions of the GUS coding sequence in scion leaves of plants of the indicated genotypes grown under short day conditions for eight weeks after grafting. Analyses were performed at HaeIII-49, MspI-126, HaeIII-1467 and MspI-1529 (see Supplementary Figure S2). The percentage of DNA methylation was calculated as described in Figure 1D. Mean and standard deviation bars are based on two biological replicates.