Table 1.
Efficiency of transmission of S-PTGS from L1 rootstocks to 6b4 scions
| Number of silenced scions/total plants | |||
|---|---|---|---|
| Grafting combination | Exp 1 | Exp 2 | % of S-PTGS transmission |
| 6b4 // L1 | 35/35 | 24/24 | 100 |
| 6b4 // L1rdr6 | 0/13 | 0/8 | 0 |
| 6b4 // L1clsy1 | 6/6 | 5/5 | 100 |
| 6b4 // L1nrpd1 | 5/5 | 6/6 | 100 |
| 6b4 // L1rdr2 | 9/9 | 8/8 | 100 |
| 6b4 // L1dcl3 | 15/15 | 7/7 | 100 |
| 6b4 // L1nrpe1 | 12/12 | 16/16 | 100 |
| 6b4 // L1drd1 | 8/8 | 8/8 | 100 |
| 6b4 // L1drm2 | 7/7 | 6/6 | 100 |
| 6b4 // L1drm2 cmt3 | 6/6 | 16/16 | 100 |
| 6b4 rdr6 // L1 | 0/8 | 0/8 | 0 |
| 6b4 drm2 // L1 | 6/6 | 22/22 | 100 |
For each grafting combination, two biological replicates were analyzed. Shoots were considered silenced when exhibiting GUS activity < 20 fluorescent units per minute per microgram of total proteins, eight weeks after grafting. The efficiency of S-PTGS transmission is expressed as the percentage of silenced plants when summing the results of two replicates.