Figure 1.

(A) Incorporation of GTP and dGTP into the initiator (F-10dA) depending on Tris and borate concentrations. (B) Time-course analysis of GTP incorporation at various borate concentrations in the Tris-based buffer, where 100:50 TB means that Tris-borate buffer is composed of 100 mM Tris and 50 mM borate in the working solution (1×). In brief, 30 μM GTP or 50 μM dGTP was applied to 3 μM initiator, which was labeled at the 5′-end with the FAM dye, with measurement of the incorporation efficiency by denaturing PAGE in a 16% gel (with 7 M urea).