Figure 3.

(A) Quantity of incorporated natural ribonucleotides, and (B) modified ribonucleotides in Tris-borate buffer compared to a conventional reaction buffer for TdT: 20 mM Tris-HCl. Each concentration of total and single nucleotides that are incorporated into ODNs was determined by measuring the relevant peak intensity after PAGE. Because 3 μM initiator (F-10dA, 10 nt) was used, the single incorporated nucleotide that matches the concentration of the initiator depicted as a dotted line means that a high yield of a single-nucleotide incorporation product (11 nt) would be obtained.