Fig. 2.

BnLPAT gene models with target sequences and schematic of binary vectors. a, b Diagram of the BnLPAT2 and BnLPAT5 gene models. Exons are shown with blue rectangles, whereas introns are shown with black lines. Target sites in the conserved CDS region of BnLPAT genes. SNPs are highlighted in light red. PAMs are coloured green, whereas gRNAs are coloured yellow. c, d Diagram of the single-gRNA- and multi-gRNA-mediated CRISPR-Cas9 constructs. The expression of the Cas9 protein is driven by the 2× 35S promoter with a 2× nuclear localization signal (NLS) at the N terminus. The Arabidopsis promoter U6-26 is used to drive the sgRNA expression