Table 2.
Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) |
---|---|---|
Human-GAPDH | GAAAGACAACCAGGCCATCAG | TCATGAATGCATCCTTTTTTGC |
Human-IL-1β | CCCTGAACTCAACTGTGAAATAGCA | CCCAAGTCAAGGGCTTGGAA |
Human-IL-6 | ATTGTATGAACAGCGATGATGCAC | CCAGGTAGAAACGGAACTCCAGA |
Human-TNF-α | TCAGTTCCATGGCCCAGAC | GTTGTCTTTGAtGATCCATGCCATT |
Mouse-GAPDH | TGTGTCCGTCGTGGATCTGA | TTGCTGTTGAAGTCGCAGGAG |
Mouse-IL-1β | TCCAGGATGAGGACATGAGCAC | GAACGTCACACACCAGCAGGTTA |
Mouse-IL-6 | CCACTTCACAAGTCGGAGGCTTA | CCAGTTTGGTAGCATCCATCATTTC |
Mouse-TNF-α | TATGGCCCAGACCCTCACA | GGAGTAGACAAGGTACAACCCATC |
PCR, polymerase chain reaction.