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. 2019 Aug 22;30(9):2384–2392. doi: 10.1021/acs.bioconjchem.9b00490

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Copyright © 2019 American Chemical Society

This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License, which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes.

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Flow cytometric analysis of EGFR-expressing A431 cells using 10 nM pG-ODN-functionalized Cetuximab, a, hybridized to a CY5-functionalized ODN, a′. (A) Antibody activity after purification using SEC, ultrafiltration, and magnetic beads. The fluorescence intensity of pG-ODN-Cetuximab-labeled A431 cells was compared with that of A431 cells incubated with only pG-ODN. (B) Labeling efficiency and (C) cross-contamination of pG-ODN noncovalently (−hν) or covalently (+hν) coupled to Cetuximab. pG-ODN-functionalized Cetuximab was incubated for 1 h with a 20 mol equiv of a competing pG-ODN sequence. MFI represents the median fluorescence intensity, and error bars represent the SD (n = 3).