Skip to main content
. 2019 Aug 22;30(9):2384–2392. doi: 10.1021/acs.bioconjchem.9b00490

Figure 5.

Figure 5

Protein G-ODN–antibody conjugates for applications in super-resolution microscopy. (A) A431 carcinoma cells are labeled with a pG-ODN–Cetuximab construct containing either a short (11 nt) or a long (20 nt) docking strand. Subsequently, the labeled cells are fixated to a glass slide, and super-resolution images are obtained using the transient binding of a short imager strand (DNA-PAINT) or using a long irreversible binder in combination with a photoswitchable dye (dSTORM). (B) DNA-PAINT super-resolution image obtained using ATTO647N-functionalized imager strands (20 000 frames, 20 Hz frame rate). A zoom-in on two defined areas shows the distribution of EGFR receptors. (C) Number of localizations observed over the course of 8 min during a DNA-PAINT acquisition using complementary (c) or non-complementary (n.c.) imager strands. (D) Super-resolution image obtained with dSTORM using a CY5-functionalized imager strand (20 000 frames, 65.5 Hz frame rate), including a zoom-in on two defined areas that show the distribution of EGFR receptors. Scale bars: 5 and 1 μm for the zoom-in images.