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Inhibition of SNHG5 changed the expression and methylation of DR4. The protein expression of DR4 was determined by Western blot (A) and the mRNA level of DR4 gene was analyzed by qRT-PCR (B). The methylation of DR4 CpG sites was analyzed by methylation-specific PCR (MSP) analysis in K562 cells (C). Data are expressed as the mean ±SD of at least 3 experiments. *** p<0.001 vs. control.
