Figure 3. A, StarBase and mirBase software were applied to predict the targeting relationship between SOX11 and miR-9-5p. B, After hepatocellular carcinoma (HCC) cells were transfected with miR-9-5p mimics or control mimics (miR mimics) for 48 h, the miR-9-5p expression level was assessed using qRT-PCR. C, Luciferase reporter assay in HCC cell lines (huh7 and SK-HEP-1) was used to evaluate the relationship between SOX11 and miR-9-5p. D, Western blot detected the protein expression of SOX11 in HCC cells transfected with miR-9-5p mimics or control mimics (miR mimics). E, After HCC cells were transfected with pcNDA-MEG3 (MEG3), control vector, MEG3 siRNA2, or control siRNA (NC siRNA), western blot was used to determine the SOX11 expression. Data are reported as means±SD. ***P<0.01 vs control group (t-test).
