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Gambogic acid and neogambogic acid inhibit MRSA biofilm formation in vitro. (A) Crystal violet assay to assess the antibioflm activity of GA and NGA against S. aureus ATCC33591 bioflm. (B) Percent reduction of S. aureus ATCC33591 biofilm after treatment with GA and NGA (8μg/mL). (C) Scanning electron microscopy images showing the structure of S. aureus ATCC33591 biofilm. Magnifications, x 2,000. P values of (***P ≤ 0.005) are considered significant.
