FIGURE 2.

Location of transposon insertion sites in the genome of M. bovis strain JF4278. Genes and their orientation are indicated by arrows. Genes found essential with ESSENTIALS are filled in red, while non-essential genes are filled in yellow (Zomer et al., 2012). Mapping of the transposon integrations sites from the transposon sequencing of the full mutant library (input samples) is represented by black, green, and red bars (each corresponding to one replicate experiment). The height of the insertion bars represents the read count per insertion. Approximately 4000 independent transposon insertion sites were detected with an average sequencing depth (reads/transposon insertion) of 6800 per transposon-junction per replicate.