TABLE 3.

Non-essential virulence-related factors in M. bovis strain JF4278.

Non-essential CDS in JF4278 (MBOVJF4278_)	VFDB hit and/or M. bovis virulence attributes	Blast score	Blast E-value
00282	IS1634AV transposase [MmmSC]	1060	0
00094	IS1634AV transposase [MmmSC]	1059	0
00280	IS1634AV transposase [MmmSC]	1025	0
00861	IS1634AV transposase [MmmSC]	1018	0
00281	NADH oxidase [M. bovis]1	926	0
00808	Variable surface lipoprotein N [M. bovis]2; (VpmaVprecursor [M. agalactiae])	870; (313)	0; (1E−85)
00132	TrmFO [M. bovis]3	859	0
00670	Membrane nuclease MnuA [M. bovis]4; (membrane nuclease [M. pulmonis])	751; (173)	0; (2E−43)
00889	P48, predicted lipoprotein [M. agalactiae]	743	0
00150	P48, predicted lipoprotein [M. agalactiae]	627	1E−180
00700	Endopeptidase Clp ATP-binding chain C [Listeria monocytogenes]	546	1E−155
00006	Oligopeptide ABC transporter [MmmSC]	414	1E−116
00429	VpmaX [M. bovis]5	353	2E−126
00819	Variable surface lipoprotein K [M. bovis]	289	2E−103
00390	PgPepO oligopeptidase [Mycobacterium sp. JLS]	280	3E−75
00450	Peptide methionine sulfoxide reductase [Neisseria meningitidis]	280	8E−76
00684	Lipid A ABC exporter [Haemophilus somnus]	273	2E−73
00453	Lipid A export ATP-binding protein MsbA [H. influenzae]	234	1E−61
00884	Putative lipoate protein ligase A [L. ivanovii subsp. ivanovii]	229	2E−60
00797	Variable surface lipoprotein H [M. bovis]2	215	7E−74
00803	Variable surface lipoprotein G [M. bovis]2	201	4E−66

Non-essential virulence-related proteins identified by VFDB for M. bovis JF4278 using BLAST. Known M. bovis virulence-related factors were identified by BLAST. ¹NADH oxidase (Zhao et al., 2017). ²Variable surface lipoproteins (Wise et al., 2011). ³TrmFO (Guo et al., 2017). ⁴Membrane nuclease MnuA (Sharma et al., 2015; Mitiku et al., 2018). ⁵VpmaX (Zou et al., 2013). Scores and E-values were generated by the BLAST algorithm. VFDB hits were filtered by BLAST scores >200.