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. 2019 Sep 13;13:416. doi: 10.3389/fncel.2019.00416

FIGURE 2.

FIGURE 2

Gentamicin reduces the respiratory control ratio (RCR) of isolated mitochondria in a dose- and time-dependent manner. (A) Increase in complex II succinate-induced O2 flux (state 4) with increasing gentamicin concentrations, and a simultaneous reduction in the CCCP-induced O2 flux (state 3u). Experiments were done in the presence of 2.5 μM rotenone, to inhibit complex I respiration. Lines are fit to a linear regression model, displaying 95% confidence intervals (N = 13, n = 3–26). (B) Transformed data, with RCRs derived by dividing the CCCP-induced O2 consumption rates (state 3u) by the succinate-induced O2 consumption rates (state 4) (EC50 3.6 mM, nH 2.4). (C) Decrease in the RCR with increasing gentamicin incubation times, for three different gentamicin concentrations (2.5, 5, and 7.5 mM) (N = 1, n = 1). (D) Dose-response curve for state 3u respiration with gentamicin at t = 10, with an IC50 of 9.5 mM (95% CI 8.4–11.0 mM, nH 1.54) (N = 1, n = 3).