FIGURE 5.

The effect of gentamicin on mitochondrial H₂O₂ (ROS) production. (A) H₂O₂ levels detected over a period of 10 min, with the rates of production and overall concentrations plotted. Solid lines display Amplex signal (H₂O₂ concentration), dotted lines display rates of H₂O₂ production. Green is control sample, red is pre-treated with gentamicin. Following succinate addition (10 mM) (+), the formation of H₂O₂ is reduced in samples that were pre-incubated for 10 min with 5 mM gentamicin. (B) Shows quantification of the increase in cumulative H₂O₂ over a 10 min period, with final concentrations plotted (N = 3, n = 3). Gentamicin reduces endogenous ROS production.