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Analysis of phrA regulation in different sugars using a LacZ reporter assay. The activity of ß-glactosidase under regulation of PphrA is expressed in nmol p-nitrophenol/min/mL (Miller Units). A strain containing promoterless pPP1 plasmid (pPP1-D39) was included as a control in the assay. Values are average of three independent experiments each with three replicates. Bars indicate standard error of mean (SEM). ** p < 0.01, *** p < 0.001, **** p < 0.0001.
