Figure 8.

Views of the TM with two-photon and AOG imaging. Note, images are inverted compared with prior figures. (A) En face autofluorescence section of the human TM in situ using two-photon imaging (reprinted by permission from Gonzalez JM Jr, Heur M, Tan JC. Two-photon immunofluorescence characterization of the trabecular meshwork in situ. Invest Ophthalmol Vis Sci. 2012;53:3395–404). The CBB is seen as dense horizontal fibers at the top of the image. TM beams are seen between the hash lines with loosely packed beams near the ciliary muscle and densely packed beams below. The cornea is seen below the bottom hash line. Hoechst-33342 labeling illustrates the TM cell nuclei. (B) AOG montage of iridocorneal angle in participant 4. The uveal meshwork beams (green arrows) are visualized coursing vertically over deeper, more densely packed corneoscleral TM beams (white arrow) running parallel to the limbus. (C) AOG montage of iridocorneal angle in participant 5. In both participants, a reflective band of the TM is visible midway up the iridocorneal angle (yellow asterisks). The densely packed corneoscleral TM beams (white arrows) are visible in both participants. In both participants densely packed corneoscleral TM beams (white arrows) run parallel to the limbus and become thinner as we move anteriorly. The beaded appearance of cellular bodies on the beams in AOG images (B, C) closely resembles that of the cell nuclei on the TM beams in panel A. Scale bars, 50 μm.