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. 2019 Sep 13;10:2127. doi: 10.3389/fmicb.2019.02127

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Copyright © 2019 Zhong, Zhang, Liu, Liu, Chen, Fu, Qi and Cao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Determination of the PolR DNA binding sequence. (A) Alignment of promoters of target genes of PolR. The conserved nucleotides are marked by a dark background. (B) The consensus DNA binding site of PolR predicted by RegPredict. (C) PolR-protected region upstream of pit (MLP_RS00235) determined by DNaseI footprinting. The control reaction (no protein) is shown in red. The reaction including 3.0 μg PolR is shown in blue. The dotted box indicates the protected region. The nucleotides protected by PolR are shown beneath the chromatograms.