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. 2019 Sep 13;10:2127. doi: 10.3389/fmicb.2019.02127

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Copyright © 2019 Zhong, Zhang, Liu, Liu, Chen, Fu, Qi and Cao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PolR binds to the promoter of genes associated with poly-P metabolism and phosphate transport in vivo. (A) The expression of PolR during growth in the SBR system was detected by Western blotting assay using anti-PolR. (B) The enrichment of the promoters of pit (MLP_RS00235, MLP_RS24590), ppgk (MLP_RS12905), ppk (MLP_RS23025), and pstS (MLP_RS23035) at stage T3 (aerobic condition, light gray) and stage T4 (anaerobic condition, dark gray) was analyzed by ChIP-qPCR assay. (C) The enrichment of the promoters of other Poly-P metabolism related genes. The relative enrichment value of each gene was normalized for the 16S rRNA gene, and the enrichment value of each gene in the input DNA samples was arbitrarily set to one. The values were obtained by three independent experiments and are shown as means ± standard deviations. ^**, compared with the data at stage T3, P < 0.01.