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Titration of hOAT with 1 to obtain a partition ratio of 12 ± 1. The number of equiv of 1 required for inactivation is derived from the x intercept (y = 0%) of the linear regression of the initial linear (first-order) relationship (x = 0–10 equiv); the non-pseudo-first-order kinetics and deviation from linearity (x = 20–30 equiv) are possibly a result of product inhibition. The partition ratio is the number of equiv of 1 for inactivation minus 1.
