Table 1.

Evaluating the effects of the microenvironment (elevated glucose or dynamic perfusion) and different chemical stimulants (capsaicin and sodium lauryl sulfate) on cell viability and gene and protein expression using the corneal tissue model. (Abbreviations: Interleukin-1 beta (IL-1β), Substance P (SP), Calcitonin gene-related peptide (CGRP); Gene names: CRCP -accessory protein for CGRP, SCN- sodium channel, KERA - keratocan (cornea-specific proteoglycan), LUM - lumican (proteoglycan abundant in the stroma)).

Chemical	Condition Applied	Response	Reference
Elevated glucose	35-55 mM Glucose	Concentration-dependent loss of hCECs and hCSSCs, nerve degeneration, ↑ IL-1β	(Deardorff et al., 2018)
Dynamic perfusion	Intraocular pressure of 15-20 mmHg and tear flow of 50 μL/min	↑ CRCP, ↑ SCN, ↑ KERA, ↑ LUM	(Siran et al., 2018)
Capsaicin	0.005% w/v in DMEM	↑ SP, ↑ CGRP	(Siran et al., 2018)
Sodium lauryl sulfate	10% v/v in PBS	↑ SP, ↑ Tissue permeability	(Siran et al., 2018)