Table 2.

Common secreted proteins related to nerve activation or tissue inflammation present in conditioned media isolated from corneal constructs. The relative concentration ranges of each protein detected in conditioned media in response to various chemical stimuli have been previously reported by our group (Deardorff et al., 2018; Siran et al., 2018). (Abbreviations: Substance P (SP), Calcitonin gene-related peptide (CGRP), Interleukin-1 beta (IL-1β), Tumor necrosis factor-alpha (TNF-α), Matrix metalloproteinase-9 (MMP-9)).

Protein	Major Cell Source	Subclass	Concentration Detected in Conditioned Media	Reference
SP	Sensory Neuron (hiNSCs)	Neuropeptide	100-4000 pg/mL	(Siran et al., 2018)
CGRP	Sensory Neuron (hiNSCs)	Neuropeptide	300-800 pg/mL	(Siran et al., 2018)
IL-1β	Epithelium (hCECs)	Pro-inflammatory cytokine	5-50 pg/mL	(Deardorff et al., 2018; Siran et al., 2018)
TNF-α	Epithelium (hCECs)	Pro-inflammatory cytokine	20-350 pg/mL	(Deardorff et al., 2018; Siran et al., 2018)
MMP-9	Epithelium (hCECs)	Matrix metalloproteinase	2000-5000 pg/mL	(Deardorff et al., 2018)