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. 2019 Sep 20;14(9):e0222943. doi: 10.1371/journal.pone.0222943

Fig 8.

Fig 8

Frequencies of αβ T cells (A) and CD8αα+ and CD8αβ+ subpopulations (B) expressing perforin (C and D). At indicated time points, three to five animals were subjected to necropsy. After preparation of single cell suspensions from nasal mucosa, BAL, lung tissue and lung lymph node, flow cytometric analyses were performed to determine frequency of CD3+/ γδTCR- T cells (A). Further classification (B) was made based on the expression of CD8αα homo- or CD8αβ heterodimers. MFI (mean fluorescence intensity) indicated cytolytic activity of CD8αα (C) and CD8αβ (D) T cells. * = p≤0.05 Kruskal-Wallis test followed by Dunn’s post hoc test compared to control. x in graph axis indicates infection.