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. Author manuscript; available in PMC: 2020 Sep 5.
Published in final edited form as: Cell. 2019 Aug 29;178(6):1452–1464.e13. doi: 10.1016/j.cell.2019.07.046

Figure 4. Loss of Aca1 repressor activity affects the transcription of the gene immediately downstream of the anti-CRISPR locus.

Figure 4.

The transcription of the indicated phage genes from wild-type JBD30 and JBD30aca1R44A during one-round of infection was determined by RT-qPCR. The genes assayed were: acrIFI (A); transposase, an early expressed gene (B); I/Z, the scaffold gene, which lies immediately downstream of aca1 (C); and G, a late gene lying directly upstream of the acr gene (D). Expression levels were normalized to the geometric mean of the transcript levels of two bacterial housekeeping genes: clpX and rpoD. The mean ± SEM of three independent experiments is shown. Assays were performed in PA14ΔCRISPR at a MOI of 8.