Skip to main content
. Author manuscript; available in PMC: 2020 Sep 5.
Published in final edited form as: Cell. 2019 Aug 29;178(6):1452–1464.e13. doi: 10.1016/j.cell.2019.07.046

Figure 5. Overexpression of Aca1 inhibits phage-borne anti-CRISPRs.

Figure 5.

(A) Tenfold dilutions of lysates of JBD30 carrying the indicated mutations in the Aca1 binding sites (IR1 and IR2) were applied to lawns of PA14 or PA14ΔCRISPR expressing wild-type Aca1 from a plasmid. A representative image of at least three replicates is shown. (B) Tenfold dilutions of phage JBD30 lysate carrying the indicated inverted repeat mutation were applied to lawns PA14 or PA14ΔCRISPR expressing the R44A Aca1 mutant from a plasmid. A representative image from three biological replicates is shown.