Video 1. A representative time-lapse movie shows Kip2-3xsfGFP molecules appearing as speckles from the SPB and moving along the cytoplasmic microtubule towards its plus-end.

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DOI: 10.7554/eLife.48627.011

All Kip2-3xsfGFP speckles reach the plus-end. The movie consists of 80 frames that were taken every 1.07 s and the frame speed is sped up by 3-fold for better visualization. Scale bar, 2 µm.