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. 2019 Sep 16;68(10):1874–1885. doi: 10.2337/db19-0308

Figure 3.

Figure 3

Cellular origins of beige adipocytes in mice born and raised at 30°C (thermoneutrality). A: AdipoChaser mice born and raised at thermoneutrality (30°C) were fed a standard chow diet until 8 weeks of age before being switched to doxycycline (Dox)-containing chow diet (600 mg/kg) for 10 days (“Pulse”). Following the pulse-labeling period, mice were switched back to the standard chow diet (no doxycycline) for 3 days before being subjected to cold exposure (6°C) or β3-adrenergic receptor agonist CL316,243 (CL) administration (10 mg/kg/day) for 7 days (“Chase”). RT, room temperature. B: Representative 4× brightfield image of UCP1 expression in inguinal WAT sections from mice following the Chase period. IHC, immunohistochemistry. C: Representative 63× image of inguinal WAT sections stained with anti-GFP (green) and anti-PERILIPIN (red) antibodies and counterstained with DAPI (blue [nuclei]) at indicated time points. Bar graphs/scatter plots (mean ± SD) depict the percentage of PERILIPIN+ cells expressing GFP at indicated time points. *P < 0.05 from Student t test. D: Pie chart summarizing the relative contribution of de novo adipogenesis vs. adipocyte activation/interconversion to the total pool of beige adipocytes originating following exposure to cold temperatures of β3-adrenergic receptor agonist. Percentages indicated represent mean values from the data shown in C.