Fig. 1.

Impact of miR-153 on insulin and dopamine secretion. After stimulation with 25 mmol/l glucose (a, b) or 50 mmol/l KCl (c, d) in MIN6 cells, or primary mouse islets transfected with scrambled control, miR-153 mimic or miR-153 inhibitor for 72 h, insulin secretion was measured. Data are presented as fold change compared with the level before stimulation. Experiments were performed six times (n=6) in triplicate. (e, f) Dopamine secretion (fold change) in PC12 cells stimulated with 25 mmol/l KCl with (e) or without (f) PMA compared with basal levels, 72 h after transfection with miR-153 mimic or miR-153 inhibitor (n=3). (g) Change in mouse islet [Ca²⁺] responses after islet transfection with scrambled controls, miR-153 mimic or miR-153 inhibitor for 72 h, followed by stimulation with 30 mmol/l KCl. *p<0.05 and **p<0.01 vs controls