Fig. 2.

Effect of miR-153 mimic or inhibitor on calcium genes: putative miR-153 target site in the Cacna1c 3′-UTR. (a) Expression analysis of miR-153 predicted targets by quantitative real-time PCR 72 h after transfection with scrambled control (black bars), miR-153 mimic (grey bars) or miR-153 inhibitor (white bars). All data were normalised to Gapdh (n=3). (b) MiR-153 predicted target site in mouse Cacna1c 3′-UTR and mutated 3′-UTR sequence with four nucleotide replacements (lower case letters). There is strong sequence conservation in mice, rats and humans. (c) Luciferase reporter assay demonstrating functional activity of miR-153 mimic on WT Cacna1c 3′-UTR in 293T cells, but not on mutated Cacna1c 3′-UTR. Normalised to Renilla luciferase activity (n=3). *p<0.05 and **p<0.01 vs controls