Fig. 3.

Regulation of Cacna1c expression by miR-153. Effects of miR-153 mimic and miR-153 inhibitor on Cacna1c expression by quantitative real-time PCR: (a) MIN6 cells; (b) mouse islets; (c) PC12 cells. (n=3). Western blots for CACNA1C and α-tubulin protein levels in MIN6 cells (d) and PC12 cells (e). The relative expression values were determined by Image J (n=3). Ia-2β mRNA levels were unchanged in MIN6 cells (f), mouse islets (g) and PC12 cells (h) following transfection with miR-153 mimic or inhibitor. (n=3), *p<0.05 and **p<0.01 vs controls