Fig. 4.

Effect of miR-153-Cacna1c target protector. Glucose stimulates insulin secretion (fold change) in MIN6 cells (a) and mouse islets (b), after transfection with miR-153 mimic with and without miR-153-Cacna1c target protector (TP), showing that the target protector partly abrogated the inhibitory effect of miR-153 on GSIS (n=3). Cacna1c expression analysis by quantitative real-time PCR in MIN6 cells (c) and mouse islets (d) after transfection with miR-153 mimic with and without its target protector. All data were normalised to Gapdh. n=3, *p<0.05 vs controls, †p<0.05, miR-153 mimic vs TP+miR-153 mimic