| Antibodies |
|
| Mouse monoclonal anti-Tnnt2 |
Thermo Fisher Scientific |
Cat#MA5-12960; RRID: AB_11000742 |
| Rabbit polyclonal anti-GFP |
Thermo Fisher Scientific |
Cat# A-11122; RRID: AB_221569 |
| Alexa fluor 647 donkey anti-mouse IgG |
Thermo Fisher Scientific |
Cat# A-31571; RRID:AB_162542 |
| Alexa fluor 488 goat anti-rabbit IgG |
Thermo Fisher Scientific |
Cat# A-11008; RRID:AB_143165 |
| Alexa fluor 555 goat anti-mouse IgG |
Thermo Fisher Scientific |
Cat# A-28180; RRID:AB_2536164 |
| Alexa fluor 488 donkey anti-rabbit IgG |
Thermo Fisher Scientific |
Cat# A-21206; RRID:AB_2535792 |
| Goat polyclonal anti-Gata4 |
Santa Cruz Biotechnology |
Cat# sc-1237; RRID: AB_2108747 |
| Goat polyclonal anti-Tbx5 |
Santa Cruz Biotechnology |
Cat# sc-17866; RRID:AB_2200827 |
| Mouse monoclonal anti-Ty1 |
Diagenode |
Cat# C15200054 |
| Rabbit polyclonal anti-H3K27ac |
Diagenode |
Cat# C15410196; RRID:AB_2637079 |
| Rabbit monoclonal anti-Mef2c |
Cell Signaling |
Cat# 5030; RRID:AB_10548759 |
| Goat polyclonal anti-Hand2 |
R&D Systems |
Cat# AF3876; RRID:AB_2295155 |
| Mouse monoclonal anti-Gapdh |
Merck Millipore |
Cat# MAB374; RRID:AB_2107445 |
| Rabbit polyclonal anti-mCherry |
Abcam |
Cat# ab167453; RRID:AB_2571870 |
|
| Bacterial and Virus Strains |
|
| One Shot™ TOP10 Chemically Competent E. coli |
Thermo Fisher Scientific |
C404010 |
| One Shot™ Stbl3™ Chemically Competent E. coli |
Thermo Fisher Scientific |
C737303 |
|
| Chemicals, Peptides, and Recombinant Proteins |
|
| Erlotinib |
Selleckchem |
S1032 |
| Ruxolitinib |
Selleckchem |
S1378 |
| Gefitinib |
Selleckchem |
ZD1839 |
| AZD1480 |
Selleckchem |
S2162 |
| B27 supplements |
Invitrogen |
17504–044 |
| SureCoat |
Cellutron |
sc9035 |
| Insulin, Transferrin, Selenium Solution |
Invitrogen |
41400045 |
| MEM Non-Essential Amino Acids Solution |
Invitrogen |
11140050 |
| MEM Vitamin Solution |
Invitrogen |
11120052 |
| MEM Amino Acids Solution |
Invitrogen |
11130051 |
| Matrigel |
Corning |
354248 |
| 4’,6-diamidino-2-phenylindole |
Thermo Fisher Scientific |
D1306 |
| Hoechst 33342, Trihydrochloride, Trihydrate |
Thermo Fisher Scientific |
H3570 |
|
| Critical Commercial Assays |
|
| ChIP-IT Express |
Active Motif |
53008 |
| BLOCK-iT U6 RNAi Entry Vector Kit |
Thermo Fisher Scientific |
K494500 |
|
| Deposited Data |
|
| Raw and analyzed data |
This paper |
GEO: GSE112315, GEO: GSE112316, GEO: GSE112317
|
|
| Experimental Models: Cell Lines |
|
| Platinum-E (Plat-E) Retroviral Packaging Cell Line |
Cell Biolabs |
RV-101 |
| HEK-293 Cell Line |
ATCC |
CRL-1573; RRID:CVCL_0045 |
|
| Experimental Models: Organisms/Strains |
|
| Mouse: αMHC-GFP |
Song et al., 2012 |
N/A |
| Mouse: C57BL/6J |
The Jackson Laboratory |
N/A |
|
| Oligonucleotides |
|
| Primer: Mouse Myh6 Forward: 5’-GCC CAG TAC CTC CGA AAG TC-3’
|
This paper |
N/A |
| Primer: Mouse Myh6 Reverse: 5’-GCC TTA ACA TAC TCC TTG TC-3’
|
This paper |
N/A |
| Primer: Mouse Tnnt2 Forward: 5’-GTA GAG GAC ACC AAA CCC AAG-3’
|
This paper |
N/A |
| Primer: Mouse Tnnt2 Reverse: 5’-GAG TCT GTA GCT CAT TCA GGT C-3’
|
This paper |
N/A |
| Primer: Mouse Gapdh Forward: 5’-AGG TCG GTG TGA ACG GAT TTG-3’
|
This paper |
N/A |
| Primer: Mouse Gapdh Reverse: 5’-TGT AGA CCA TGT AGT TGA GGT CA-3’
|
This paper |
N/A |
| Jak2 shRNA sequence: 5’- GGTGGAATTCAGTGG TCAAGAcgaaTCTTGACCACTGAATTCCACC-3’
|
This paper |
N/A |
| LacZ shRNA sequence: 5’- CTACACAAATCAGCG ATTTcgaaAAATCGCTGATTTGTGTAG-3’
|
This paper |
N/A |
|
| Recombinant DNA |
|
| pMXs-GW |
Addgene |
#18656; RRID:Addgene_18656 |
| pMXs-puro |
Cell Biolabs, Inc. |
RTV-012 |
| pMXs-Mef2c-3xTy1 |
This paper |
N/A |
| pMXs-3xTy1-Hand2 |
This paper |
N/A |
| pBabeX-Gata4 |
Song et al., 2012 |
N/A |
| pBabeX-Hand2 |
Song et al., 2012 |
N/A |
| pBabeX-Mef2c |
Song et al., 2012 |
N/A |
| pBabeX-Tbx5 |
Song et al., 2012 |
N/A |
| Gja5 enhancer-Hsp68-LacZ |
This paper |
N/A |
| Tnni1 enhancer-Hsp68-LacZ |
This paper |
N/A |
| pMXs-puro-Gja5 enhancer-Hsp68-mCherry |
This paper |
N/A |
| pMXs-puro-Tnni1 enhancer-Hsp68-mCherry |
This paper |
N/A |
| pRS-Egfr shRNA |
ORIGENE |
TR509941 |
| pMXs-Jak2 shRNA |
This paper |
N/A |
|
| Software and Algorithms |
|
| FlowJo v10.5.3 |
FLOWJO, LCC |
https://www.flowjo.com/ |
| Cytoscape v3.6.0 |
Institute for Systems Biology |
https://cytoscape.org/ |
| R v3.4.1 |
The R Foundation |
https://www.r-project.org/foundation/ |
| MACS v2.1.0 |
Feng et al., 2012 |
http://liulab.dfci.harvard.edu/MACS/ |
| GREAT |
McLean et al., 2010 |
http://great.stanford.edu/public/html/ |
| BEDTools v2.26.0 |
Quinlan and Hall, 2010 |
https://bedtools.readthedocs.io/en/latest/ |
