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. Author manuscript; available in PMC: 2020 Sep 19.
Published in final edited form as: Cell Chem Biol. 2019 Jun 27;26(9):1253–1262.e5. doi: 10.1016/j.chembiol.2019.05.011

Figure 4. Localization of β’-COP and GGA1 with Exo-Erythrocytic Forms During Liver-Stage Plasmodium Infection.

Figure 4.

(A and B) Huh7 cells were infected with P. berghei-Luc sporozoites and fixed at 3, 24 and 48 h post-infection. Cell were stained with anti-β’ COP (A, red) or anti-GGA1 (B, red), and anti-PbHSP70 (green) to visualize Plasmodium. Nuclei were stained with DAPI (blue). Scale bars, 10 μm. See Figure S4A for GGA1-stained uninfected cells at 24 and 48 hpi.

(C) Representative confocal images of P. berghei-infected Huh7 cells at 48 hpi. β’-COP (red, top panel) and GGA1 (red, bottom panel) colocalize with PbUIS4 (green, both panels), a PVM-resident protein. Nuclei were stained with DAPI. Scale bars, 10 μm.

(D) Hepa1-6 cells were infected with P. yoelii sporozoites and fixed at 48 hpi. Cells were stained with anti-β’ COP (red, top panel) or anti-GGA1 (red, bottom panel), and anti-PbHSP70 (green, both panels). Nuclei were stained with DAPI (blue). Scale bars, 20 μm.