Figure 1. The effect of 2–5AMD and RNase L activation on polysomes in A549 human cells.

(A) Schematic overview of the dsRNA sensing pathways and the location of 2–5AMD. (B) Polysome sedimentation profiles in WT and RNase L^−/− cells during poly I:C treatment. (C) Translation activity in WT and RNase L^−/− cells after poly I:C transfection measured using ³⁵S metabolic labeling (lower panels). Total proteins stained with coomassie show lane loading (upper panels). Independent measurements using an orthogonal readout are shown in related Fig. S1. (D) Quantification of new protein synthesis, normalized to loading control. Error bars are S.E. from three biological replicates (Fig. 1C, Fig. S1A–B).